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KMID : 0624620080410090670
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BMB Reports
2008 Volume.41 No. 9 p.670 ~ p.677
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Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus
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Pongsomboon Siriporn
Tang Sureerat
Boonda Suleeporn
Aoki Takashi
Hirono Ikuo
Yasuike Motoshige
Tassanakajon Anchalee
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Abstract
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A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly (≤ 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by realtime RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.
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KEYWORD
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DNA microarray, Gene expression, Penaeus monodon, Real-time RT-PCR, Yellow head virus
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